Crystallization (Comment) | Organism |
---|---|
by vapour diffusion using a protein solution with 2 mM protein in 10 mM Tris, pH 7.5, 2 mM CaCl2, against a precipitation solution containing 11% PEG 4000 in 0.1 M MES, pH 6.5, at room temperature, X-ray diffraction structure determination and analysis at 2.2 A resolution. Comparison with structures of enzyme mutants, overview | Loligo vulgaris |
Protein Variants | Comment | Organism |
---|---|---|
additional information | reengineering of DFPase through rational design to bind and productively orient the more toxic SP stereoisomers of the nerve agents sarin and cyclosarin, creating a modified enzyme with enhanced overall activity and significantly increased detoxification properties | Loligo vulgaris |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | dependent on | Loligo vulgaris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
x * 35000 | Loligo vulgaris |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
diisopropyl fluorophosphate + H2O | Loligo vulgaris | - |
diisopropyl phosphate + fluoride | - |
? | |
additional information | Loligo vulgaris | diisopropyl fluorophosphatase acts on a variety of organophosphorus compounds | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Loligo vulgaris | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diisopropyl fluorophosphate + H2O | - |
Loligo vulgaris | diisopropyl phosphate + fluoride | - |
? | |
additional information | diisopropyl fluorophosphatase acts on a variety of organophosphorus compounds | Loligo vulgaris | ? | - |
? | |
additional information | The enzyme also acts as Ca2+-dependent phosphotriesterase. The hydrolytic reaction catalyzed by DFPase leads to the formation of a phosphate or phosphonate and a fluoride ion, resulting in detoxification of the organophosphorus agent. Presence of a phosphoenzyme intermediate in the reaction mechanism, which involves direct nucleophilic attack by Asp229 on the substrate, but no metal-assisted water activation, overview | Loligo vulgaris | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 35000 | Loligo vulgaris |
Synonyms | Comment | Organism |
---|---|---|
DFPase | - |
Loligo vulgaris |
diisopropyl fluorophosphatase | - |
Loligo vulgaris |
General Information | Comment | Organism |
---|---|---|
physiological function | diisopropyl fluorophosphatase is a calcium-dependent phosphotriesterase that acts on a variety of highly toxic organophosphorus compounds, that act as inhibitors of acetylcholinesterase | Loligo vulgaris |